recoveries more than 100% [Bioanalytics]

posted by deepakpangavhane – India, 2018-09-03 08:28 (1446 d 00:39 ago) – Posting: # 19205
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Hi everyone!

The % recovery was 107.80%, 112.65% and 105.92% for the low quality control (LQC) and middle quality controls (MQC2 and MQC1), respectively. Whereas % recovery for higher quality control (HQC) was 98.25%.

The % recovery was determined by comparing the mean peak area of 6 replicates of extracted quality control samples at High, Middle and Low concentrations against respective mean peak area of 6 replicates of un-extracted quality control samples at High, Middle and Low concentrations. Internal standard used was deuterated.

Kindly confirm recoveries markedly higher than 100% for LQC, MQC2 and MQC1 are acceptable as per USFDA guidelines?? Also specify reason for higher recoveries.

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