recoveries more than 100% [Bioanalytics]

posted by Ladi – Thailand, 2018-09-04 03:51  – Posting: # 19232
Views: 2,773

Hello Deepak and Ohlbe,

» The % recovery was 107.80%, 112.65% and 105.92% for the low quality control (LQC) and middle quality controls (MQC2 and MQC1), respectively. Whereas % recovery for higher quality control (HQC) was 98.25%.

My lab called the 'post-extraction' as 'un-extracted', I am guessing you also mean that?

I observed recovery 100-115% mostly with protein precipitation projects in our lab. My explanation to auditors was that the extracted samples are little bit more concentrated than the post-extracted samples because some protein is precipitated out in extracted samples. While in the post-extracted samples, the measured volume of supernatant from precipitated blank is use to make up a required concentration. Ususally for protein precipitation, recovery is almost 100%. So if extracted is more concentrated than it supposed to be (expected conc.), recovery may cross 100%. However, I normally see similar %recovery across all concentrations.

Regards,
Ladi

Complete thread:

Activity
 Admin contact
20,646 posts in 4,328 threads, 1,436 registered users;
online 13 (1 registered, 12 guests [including 5 identified bots]).
Forum time: 21:19 CEST (Europe/Vienna)

No one should approach the temple of science
with the soul of a money changer.    Thomas Browne

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5