Log in
Registerrecoveries more than 100% [Bioanalytics]
Hello Deepak and Ohlbe,
» The % recovery was 107.80%, 112.65% and 105.92% for the low quality control (LQC) and middle quality controls (MQC2 and MQC1), respectively. Whereas % recovery for higher quality control (HQC) was 98.25%.
My lab called the 'post-extraction' as 'un-extracted', I am guessing you also mean that?
I observed recovery 100-115% mostly with protein precipitation projects in our lab. My explanation to auditors was that the extracted samples are little bit more concentrated than the post-extracted samples because some protein is precipitated out in extracted samples. While in the post-extracted samples, the measured volume of supernatant from precipitated blank is use to make up a required concentration. Ususally for protein precipitation, recovery is almost 100%. So if extracted is more concentrated than it supposed to be (expected conc.), recovery may cross 100%. However, I normally see similar %recovery across all concentrations.
Regards,
Ladi
» The % recovery was 107.80%, 112.65% and 105.92% for the low quality control (LQC) and middle quality controls (MQC2 and MQC1), respectively. Whereas % recovery for higher quality control (HQC) was 98.25%.
My lab called the 'post-extraction' as 'un-extracted', I am guessing you also mean that?
I observed recovery 100-115% mostly with protein precipitation projects in our lab. My explanation to auditors was that the extracted samples are little bit more concentrated than the post-extracted samples because some protein is precipitated out in extracted samples. While in the post-extracted samples, the measured volume of supernatant from precipitated blank is use to make up a required concentration. Ususally for protein precipitation, recovery is almost 100%. So if extracted is more concentrated than it supposed to be (expected conc.), recovery may cross 100%. However, I normally see similar %recovery across all concentrations.
Regards,
Ladi
Complete thread:
- recoveries more than 100% deepakpangavhane 2018-09-03 08:28 [Bioanalytics]
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- recoveries more than 100% Ohlbe 2018-09-03 19:21
- recoveries more than 100%Ladi 2018-09-04 03:51
- recoveries more than 100% Kintan Patel 2018-11-29 11:48
- Origin of figures ? Ohlbe 2018-11-30 18:23
- Origin of figures ? Kintan Patel 2018-12-05 10:04
- Origin of figures ? Ohlbe 2018-12-05 10:30
- Origin of figures ? Kintan Patel 2018-12-05 10:04
- Origin of figures ? Ohlbe 2018-11-30 18:23
- recoveries more than 100% Kintan Patel 2018-11-29 11:48
Ing. Helmut Schütz