recoveries more than 100% [Bioanalytics]
❝ The % recovery was 107.80%, 112.65% and 105.92% for the low quality control (LQC) and middle quality controls (MQC2 and MQC1), respectively. Whereas % recovery for higher quality control (HQC) was 98.25%.
My lab called the 'post-extraction' as 'un-extracted', I am guessing you also mean that?
I observed recovery 100-115% mostly with protein precipitation projects in our lab. My explanation to auditors was that the extracted samples are little bit more concentrated than the post-extracted samples because some protein is precipitated out in extracted samples. While in the post-extracted samples, the measured volume of supernatant from precipitated blank is use to make up a required concentration. Ususally for protein precipitation, recovery is almost 100%. So if extracted is more concentrated than it supposed to be (expected conc.), recovery may cross 100%. However, I normally see similar %recovery across all concentrations.
- recoveries more than 100% deepakpangavhane 2018-09-03 08:28 [Bioanalytics]