recoveries more than 100% [Bioanalytics]

posted by Ladi – Thailand, 2018-09-04 03:51 (751 d 19:46 ago) – Posting: # 19232
Views: 3,164

Hello Deepak and Ohlbe,

» The % recovery was 107.80%, 112.65% and 105.92% for the low quality control (LQC) and middle quality controls (MQC2 and MQC1), respectively. Whereas % recovery for higher quality control (HQC) was 98.25%.

My lab called the 'post-extraction' as 'un-extracted', I am guessing you also mean that?

I observed recovery 100-115% mostly with protein precipitation projects in our lab. My explanation to auditors was that the extracted samples are little bit more concentrated than the post-extracted samples because some protein is precipitated out in extracted samples. While in the post-extracted samples, the measured volume of supernatant from precipitated blank is use to make up a required concentration. Ususally for protein precipitation, recovery is almost 100%. So if extracted is more concentrated than it supposed to be (expected conc.), recovery may cross 100%. However, I normally see similar %recovery across all concentrations.

Regards,
Ladi

Complete thread:

Activity
 Admin contact
21,074 posts in 4,394 threads, 1,468 registered users;
online 2 (0 registered, 2 guests [including 2 identified bots]).
Forum time: Thursday 23:37 CEST (Europe/Vienna)

Enlightenment is man’s emergence from his self-imposed nonage
for which he himself was responsible.
Nonage is the inability to use one’s own intellect
without the direction of another.    Immanuel Kant

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5