Bioequivalence and Bioavailability Forum

Hello All,
We are trying to evaluate the tigecycline test fromulations disposition by modelling the effect of anticoagulant EDTA & HEPARIN.
Briefly,
Two different anticoagulants were tested EDTA and Heparin and it’s known that tigecycline belongs to tetracycline group of antibiotics and has strong metal ions binding potential, so by the use of EDTA and Heparin as an anticoagulant the number of metal ions released in the blood sample when it comes in contact with any of the anticoagulant. This effect was observed more with the EDTA anticoagulant. Now my point is how do we account for ex vivo Blood/plasma ratio results and in vitro protein binding into the pharmacokinetic model to explain this entire process

Any help would be greatly appreciated,
Regards
HK

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Edit: Identical post of 2015-11-16 deleted. Relax. [Helmut]