ssk
●    

2006-10-12 13:20
(6376 d 22:09 ago)

Posting: # 309
Views: 8,215
 

 sample centrifugation [Study Per­for­mance]

what are the parameters of sample centrifugation to be followed unless the sponser specifies it.
Jaime_R
★★  

Barcelona,
2006-10-12 14:04
(6376 d 21:25 ago)

@ ssk
Posting: # 310
Views: 6,835
 

 sample centrifugation

Hi ssk!

❝ what are the parameters of sample centrifugation to be followed unless the

❝ sponser specifies it.


The ones which give you good separation of plasma in the shortest possible time ;-)

In a cooled centrifuge 1500×g for 15 minutes is a rule of thumb (you have to calculate rev/min basing on the rotor's diameter).

PS: be polite!

Regards, Jaime
ssk
●    

2006-10-12 14:29
(6376 d 21:01 ago)

@ Jaime_R
Posting: # 312
Views: 6,868
 

 sample centrifugation

Sir/Madam,
What does the g stand for. What is the standard temperature at which the centrifugation usually done. How are these parameters calculated by the sponsor (rpm, temperature and the duration).
Thanking you,
ssk
Jaime_R
★★  

Barcelona,
2006-10-12 15:15
(6376 d 20:14 ago)

@ ssk
Posting: # 315
Views: 6,938
 

 sample centrifugation

Hi ssk!

❝ What does the g stand for.


g: approximately 9.81 m/s².
It's good practice to state something like '1500 × g' (1500times acceleration due to earth's gravity) in order to be able to compare different centrifuges.
Stating rpm alone means nothing (since the diameter of the rotor is not given).

For a conversion have a look at this document or this nomogram.

❝ What is the standard temperature at which the centrifugation usually done.


Cooled ;-)
Actually you don't know the temperature of the sample, but it's quite common to set the temperature of the centrifuge to +4 °C.

❝ How are these parameters calculated by the sponsor (rpm,temperature and the duration).


As already stated it should allow for easy separation of plasma.
I don't believe that sponsors do calculate anything - mainly they are skillful in 'copy-and-paste'... ;-)

Regards, Jaime
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