selwa ts ☆ Algeria, 2021-11-17 12:04 (1082 d 18:46 ago) Posting: # 22673 Views: 2,930 |
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Hello dear community, I would like to know if we are called to use HPLC or UPLC MS columns in normal phase in bioanalysis? Thanks in Advance |
Helmut ★★★ Vienna, Austria, 2021-11-17 12:43 (1082 d 18:07 ago) @ selwa ts Posting: # 22674 Views: 2,363 |
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Hi Selwa, ❝ I would like to know if we are called … Nobody calls you to use anything. You can use any stationary phase (normal or reversed phase, diol, amino, ion-exchange, …) you like. As long as the method passes validation, all is good.* ❝ … to use HPLC or UPLC MS columns in normal phase in bioanalysis? I would avoid normal phase if ever possible, since strongly non-polar mobile phases (e.g., hexane) in analytical grade are rather expensive and environmentally problematic. I suggest HILIC instead, i.e., an RP column and a mobile phase consisting of a moderately non-polar (e.g., acetonitrile) component modified by adding <20% water. Pretty old approach.3,4 In my CRO we used it for amitriptyline/nortriptyline, clobutinol, and doxepin.
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selwa ts ☆ Algeria, 2021-11-17 16:11 (1082 d 14:38 ago) @ Helmut Posting: # 22675 Views: 2,244 |
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Thank you for your quick and precise reactivity Best Regards Selwa ts |