SanTo
☆    

Indonesia,
2019-05-03 02:55

Posting: # 20268
Views: 728
 

 Bioanalysis method of iso­sorbide mono­nitrate [Bioanalytics]

Hi,

We have tried to develop a bioanalysis method of isosorbide mononitrate but it has not been successful. When tuning with LC MSMS, the parent ion intensity is e7 while the intensity of the daughter ion is e5. What we have done is with negative ESI at m / z 249.92> 59, with column C18, the mobile phase used is methanol and ammonium acetate.
Our target is 3.5 ng / mL in human plasma but when injection of samples in the solvent (not extracted) the area is not present.

Please help solve our problem. Thanks.
cakhatri
★    

India,
2019-05-28 09:13

@ SanTo
Posting: # 20303
Views: 474
 

 Bioanalysis method of iso­sorbide mono­nitrate

Dear SanTo,

The molecular weight seems to be 191.139 g/mol. How did you get 249.92 for parent compound.

Regards
Chirag
SanTo
☆    

Indonesia,
2019-06-17 11:15

@ cakhatri
Posting: # 20328
Views: 309
 

 Bioanalysis method of iso­sorbide mono­nitrate

Dear cakhatri,

The formation of the ion was observed in negative mode corresponding to an adduct with the acetate anion (M + acetate)- of m/z 249.9, whose abundance is higher than deprotonated molecular ion (M-H)- of m/z 190.

Regards

SanTo
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