konkous
☆    

Greece,
2012-09-24 21:05
(4631 d 08:07 ago)

Posting: # 9244
Views: 1,761
 

 Blank signal [Bioanalytics]

Post reply
Dear forum members

I'm currently validating a method for the determination of diclofenac in human plasma. So far i have three valid pre-study runs. During the last run i got a really strong signal for my analyte (LC/MS/MS method) equal to my LLOQ but no signal for the IS. I have no signal at the zero sample for the analyte and no signal at the blank solvent sample following the ULOQ. The most probable reason for that is a sample mix-up during pre-treatment. My question is whether to reject the run or somehow provide a reason for the high signal?

Thank you

Constantinos
 
UA Flag
Activity
 Admin contact
23,424 posts in 4,927 threads, 1,684 registered users;
49 visitors (0 registered, 49 guests [including 17 identified bots]).
Forum time: 05:12 CEST (Europe/Vienna)

Nerds don’t just happen to dress informally.
They do it too consistently.
Consciously or not, they dress informally
as a prophylactic measure against stupidity.    Paul Graham

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5