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Compliance ★ India, 2012-07-24 11:59 (4692 d 19:26 ago) Posting: # 8974 Views: 4,144 |
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Dear Sir, In the bioanalytical batch organization, most of the time it is observed that the scientist use to inject two calibration curve in a single batch. Among this two curve, one is injected at the beginning of the sequence and the second one is injected at the end of the sequence. The estimation of y=m x + C done by taking the ratio of this two curve. (If any of the one standard fail in first curve and same standard is meting the acceptance in second curve then that point would be the single other wise ratio would be used for y= m x + c determination). In this pattern, I have some query about the estimation of linearity as I feel we are using virtual value which is determined by the ratio of two observations. Does it acceptable? Can’t we plot total 16 point and derive the value of y=m x + C? Kindly come up with your expert comment. Regards, Compliance |
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Helmut ★★★   Vienna, Austria, 2012-07-24 18:16 (4692 d 13:10 ago) @ Compliance Posting: # 8976 Views: 3,313 |
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Dear Compliance, I do not understand what you (or the analyst) mean by “taking the ratio of this two curve”. ❝ Can’t we plot total 16 point and derive the value of y=m x + C? Exactly. Do be honest I have never ever (!) seen anything else. — Dif-tor heh smusma 🖖🏼 Довге життя Україна! ![[image]](https://static.bebac.at/pics/Blue_and_yellow_ribbon_UA.png) Helmut Schütz ![[image]](https://static.bebac.at/img/CC by.png) The quality of responses received is directly proportional to the quality of the question asked. 🚮 Science Quotes |
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ElMaestro ★★★ Denmark, 2012-07-25 06:58 (4692 d 00:28 ago) @ Compliance Posting: # 8977 Views: 3,302 |
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Hi Compliance, ❝ The estimation of y=m x + C done by taking the ratio of this two curve. (If any of the one standard fail in first curve and same standard is meting the acceptance in second curve then that point would be the single other wise ratio would be used for y= m x + c determination). Like HS, I also don't get the ratio thing. Did you mean average? Or did you mean pooling/using all data and getting a max likelihood fit? The latter is the right approach, I think. Anyways, the comment in parentheses quoted above sounds very very dubious to me. It sounds like someone will be cherry-picking for passing data points along the calibration curve. — Pass or fail! ElMaestro |
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Compliance ★ India, 2012-07-25 11:28 (4691 d 19:57 ago) @ ElMaestro Posting: # 8978 Views: 3,259 |
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Dear Both, Let me correct my part in my main post: it is not RATIO it is AVERAGE. Sorry for the mistake. with addition to this, I am sure that at some of the places average (mean)is taken. The software use in bio analytical(lims)gives the point by taking average of the two point. This case only insist me to raise a question on this portal. Regards, Compliance |
Ing. Helmut Schütz