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auditor ★ India, 2012-01-09 10:56 (5285 d 01:19 ago) Posting: # 7879 Views: 3,150 |
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Dear All, How the increment in the sample volume can increasing the method sensitivity. kindly explain me as i am unable to understand that during validation X concentration gives good results with Y sample volume and during study due to this Y sample volume sensitivity is affected. kindly clear me on that. Regards, Auditor |
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Helmut ★★★   Vienna, Austria, 2012-01-09 18:08 (5284 d 18:07 ago) @ auditor Posting: # 7884 Views: 2,603 |
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Dear Auditor! ❝ How the increment in the sample volume can increasing the method sensitivity. Increasing the sample volume will increase sensitivity only if you have a quite good sample preparation / trace enrichment. For simple point-and-shoot methods (e.g. protein precipitation) most likely background and/or matrix effects will increase as well outweighing the enhanced signal of the analyte(s). Since separation of analyte(s) from matrix’ components is never perfect, even in a ‘good’ method doubling the sample volume likely will not half the LLOQ. ❝ […] i am unable to understand that during validation X concentration gives good results with Y sample volume and during study due to this Y sample volume sensitivity is affected. Sorry, I don’t understand your point. Can you please reword it? — Dif-tor heh smusma 🖖🏼 Довге життя Україна! ![[image]](https://static.bebac.at/pics/Blue_and_yellow_ribbon_UA.png) Helmut Schütz ![[image]](https://static.bebac.at/img/CC by.png) The quality of responses received is directly proportional to the quality of the question asked. 🚮 Science Quotes |
Ing. Helmut Schütz