Log in
Register|
kaushal09 ☆ India, 2010-02-22 11:24 (5970 d 10:30 ago) Posting: # 4804 Views: 3,700 |
|
|
Dear All, We are using 50% variation of mean IS area of passed Calibration standard and QC for Internal standard variation calculation during analysis. Now have taken Dutraited Internal Standard. So which criteria we have consider for Internal standard variation. 50% variation can we considered ? Is there any guidance for that? Thanks in advance.  Regards, Kaushal |
|
Ohlbe ★★★ France, 2010-02-22 12:14 (5970 d 09:41 ago) @ kaushal09 Posting: # 4805 Views: 3,046 |
|
|
Dear Kaushal, No guidance that I know of, whether you're using a deuterated IS or any other IS. Suggestion: look at the variability you have during the pre-study validation. Regards Ohlbe — Regards Ohlbe |
|
kaushal09 ☆ India, 2010-02-22 13:08 (5970 d 08:47 ago) @ Ohlbe Posting: # 4807 Views: 3,056 |
|
|
Dear Ohlbe, We are using both, if available than deuterated IS otherwise another IS used. same IS variation criteria can we used for both type of Internal Standard? Regards, kaushal |
|
Ohlbe ★★★ France, 2010-02-22 13:52 (5970 d 08:02 ago) @ kaushal09 Posting: # 4810 Views: 2,993 |
|
|
Dear Kaushal, As there is no guideline you can use whatever criteria you feel are suitable. Once again, have a look at the variability you have in your pre-study validation (particularly at the full-scale precision and accuracy run, if you have one, and at matrix effects). This will help you to adapt your acceptance criteria for each method, if needed. Regards Ohlbe — Regards Ohlbe |
Ing. Helmut Schütz