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Srinivasa reddy.K ☆ Mumbai, India, 2009-09-14 15:25 (6126 d 08:09 ago) Posting: # 4198 Views: 3,854 |
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Dear sir, I wish to store few of human whole blood study samples in -70 degrees deep freezer, but i have a concern, as if the stored samples of whole blood in -70 degrees gets haemolysed. so is it a concern and if so how to address this issue? Thanks for the reply in advance. Cheers, Srinivasa reddy.K Mumbai, India. |
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Ohlbe ★★★ France, 2009-09-15 00:11 (6125 d 23:24 ago) @ Srinivasa reddy.K Posting: # 4201 Views: 3,148 |
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Dear Srinivasa, ❝ Dear sir, We also have a few ladies visiting the forum. Don't discourage them answering you  ❝ I have a concern, as if the stored samples of whole blood in -70 degrees gets haemolysed. Yes, they will get haemolysed, unless you take some very special precautions (additives, preservatives, speed of freezing, etc.), which may bring some problems for further analysis of the samples (matrix effects for instance). So unless you specifically have to work on the cells themselves, what is your concern precisely ? Enzymes being liberated from the red blood cells ? Well, if you are working on whole blood, I suppose it is because your drug is predominantly distributed in the red blood cells anyway, so they will already be in contact. Impact on the sample processing during analysis ? Your blood will be haemolysed during sample extraction in any case. Regards Ohlbe — Regards Ohlbe |
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Srinivasa reddy.K ☆ Mumbai, India, 2009-09-15 07:30 (6125 d 16:05 ago) @ Ohlbe Posting: # 4202 Views: 3,101 |
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Dear Ohlbe, Thanks for the earnest and useful reply. Well i would be very glad to encourage more ladies joining the forum. On a lighter note lets cheer up Thanks, Srinivasa reddy.k Mumbai, India. |
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