satishkoya
☆    

2009-04-04 16:35
(6296 d 20:25 ago)

Posting: # 3465
Views: 3,251
 

 ISTD variation in selectivity experiment [Bioanalytics]

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Dear sir,
Is there any specific guidelines for internal standard variation in selectivity experiment, I have processed & injected 6 replicates of LOQ samples, out of 6 replicates in 4 samples ISTD response was observed 1.25 lakhs and rest of 2 samples ISTD responce was observed 3 lakhs, please clarify how to handle this type of situation.

Regards
K.sathis & venkat

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Edit: Category changed. Explanation for non-Indians: 1 lakh = 105.
Full quote removed. Helmut
 
Ohlbe
★★★

France,
2009-04-05 01:35
(6296 d 11:24 ago)

@ satishkoya
Posting: # 3468
Views: 2,617
 

 ISTD variation: matrix effects ?

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Dear K.sathis & venkat,

I suppose that you tested 6 different sources of matrix. If you are using LC/MS/MS, you should pay a specific attention to matrix effects investigation, and if possible determine the matrix factor in the same 6 lots of matrix. This could be the explanation for such a variability, and then a source of trouble with your method.

Regards
Ohlbe
 
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