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dixit ★ India, 2009-02-13 16:27 (6338 d 09:16 ago) Posting: # 3234 Views: 3,401 |
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Dear all, Can any one tell me, on what basis does the selection of buffer in centrifuged and separated serum/ or plasma samples in BA/BE studies depend. Please guide me with literature if possible. REGARDS, DIXIT.  -- Edit: Category changed. [Helmut] |
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Ohlbe ★★★ France, 2009-02-14 17:24 (6337 d 08:19 ago) @ dixit Posting: # 3239 Views: 2,671 |
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Dear Dixit, I'm afraid I can't understand your question. What buffer are you talking about ? Regards Ohlbe |
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dixit ★ India, 2009-02-16 07:48 (6335 d 17:55 ago) @ Ohlbe Posting: # 3246 Views: 2,677 |
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Dear Ohlbe For some drugs after the blood samples are centrifuged stabilising buffers are being added to plasma samples. So I would like to the criteria for buffer selection. Regards  Dixit |
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Helmut ★★★   Vienna, Austria, 2009-02-16 15:39 (6335 d 10:04 ago) @ dixit Posting: # 3250 Views: 2,718 |
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Dear Dixit! ❝ For some drugs after the blood samples are centrifuged stabilising buffers are being added to plasma samples. … and for some drugs even immediately after blood harvesting (before centrifugation). ❝ So I would like to the criteria for buffer selection. The one preventing degradation.  Seriously: Check the literature… instead of recreating the wheel. Examples are AgNO3 for ISDN, H3PO4 for ASA, CH3COOH for cefaclor, citrate for erythromycin, N-ethylmaleimide for captopril,… Anyhow: You have to check the suitability of the stabiliser during development of the analytical method (haemolysis, chromatographic interference, matrix effect,…) — Dif-tor heh smusma 🖖🏼 Довге життя Україна! ![[image]](https://static.bebac.at/pics/Blue_and_yellow_ribbon_UA.png) Helmut Schütz ![[image]](https://static.bebac.at/img/CC by.png) The quality of responses received is directly proportional to the quality of the question asked. 🚮 Science Quotes |
Ing. Helmut Schütz