yes_naresh
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2009-12-28 06:44
(5595 d 09:11 ago)

Posting: # 4529
Views: 7,993
 

 Steady state analysis (WinNonlin) [Software]

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Dear All,

I have to perform Steady state analysis in WinNonlin, but I am not sure about the method which I supposed to be used will give correct results or not,

If any one can provide data with results then I can crosscheck with my method.


Thanks and Regards

Naresh

Edit: Category and subject line changed. [Helmut]
 
Helmut
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2009-12-30 15:45
(5593 d 00:10 ago)

@ yes_naresh
Posting: # 4539
Views: 7,984
 

 Steady state analysis (WinNonlin)

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Dear Naresh!

❝ I have to perform Steady state analysis in WinNonlin, but I am not sure

❝ about the method which I supposed to be used will give correct results or

❝ not,


Any specific questions? Which version of WinNonlin are you using (WinNonlin 5.x, Phoenix 6.x)?
I guess you have read the User's Guide (WNL v5.2.1 page 172, Phoenix 6.0 page 280, Phoenix 6.1 page 277).

❝ If any one can provide data with results then I can crosscheck with my

❝ method.


Critical are only time deviations of samples scheduled at t=tau. WinNonlin estimates AUC_TAU based on lambda_z; therefore if the last sampling time point deviates from tau you should get an AUC_TAU # AUClast.
Open WinNonlin's 'Exp1.pwo':
in 'Dosing Regimen' check ☑ 'Steady state', Dose 1, Time of Last Dose 0, Tau 24
in 'Model Options' > 'NCA Settings' > 'Calculation Method' > Linear Trapezoidal (Linear/Log Interpolation)
You should get
  Lambda_z 0.0646
  AUClast 69.7739
  AUC_TAU 69.7739 (AUCtau=AUClast, because tlast=tau)
Change the last time point in 'Exp1.pwo' from 24 h to 23.5 h > in 'NCA Workbook' > right click > Refresh
  Lambda_z 0.0677
  AUClast 68.8564
  AUC_TAU 69.4879 (AUCtau>AUClast, because tlast<tau)
Change the last time point in 'Exp1.pwo' from 24 h to 24.5 h > in'NCA Workbook' > right click > Refresh
  Lambda_z 0.0596
  AUClast 70.7462
  AUC_TAU 70.0202 (AUCtau<AUClast, because tlast>tau)

Does this example answer your question?
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yes_naresh
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2010-01-11 09:40
(5581 d 06:15 ago)

@ Helmut
Posting: # 4585
Views: 6,775
 

 Steady state analysis (WinNonlin)

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Dear HS,

Thanks for your reply; I will get back to you after going through the referred posts.

Thanks and Regards,

Naresh Shirsath
 
yes_naresh
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2010-01-13 13:58
(5579 d 01:58 ago)

@ Helmut
Posting: # 4593
Views: 6,757
 

 Steady state analysis (WinNonlin)

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Dear HS,

❝ Any specific questions? Which version of WinNonlin are you using (WinNonlin 5.x, Phoenix 6.x)?


I am using WinNonlin 5.2,

❝ I guess you have read the User's Guide (WNL v5.2.1 page 172, Phoenix 6.0 page 280, Phoenix 6.1 page 277).

❝ in 'Dosing Regimen' check ☑ 'Steady state', Dose 1, Time of Last Dose 0, Tau 24

❝ in 'Model Options' > 'NCA Settings' > 'Calculation Method' > Linear Trapezoidal (Linear/Log Interpolation)


I have read User's Guide (WinNonlin5.2) but not get required answer.

Below I have given data of 1st subject of period 1 only. I have used above steps,
DATA -
Time Point  Concentration
      0           0
     12          28.293
     24          45.195
     36          50.02
     48          49.943
     60          43.504
     72          54.623
     84          49.127
     96          55.6
     96.5        47.275
     97          43.922
     97.5        41.548
     98          55.969
     98.5        45.51
     99          54.26
     99.5        76.275
    100          76.665
    100.5        82.121
    101          75.479
    101.5        81.925
    102          93.584
    104          75.287
    108          52.053
    112          33.08
    114          27.296
    120          12.486
    132           5.6

'Dosing Regimen' check ☑ 'Steady state', Dose 35, Time of Last Dose 96, Tau 12
in 'Model Options' > 'NCA Settings' > 'Calculation Method' > Linear Trapezoidal (Linear/Log Interpolation)

Results I got,
Cmin        41.548
AUCtau     801.3215
AUClast   1259.8256
Lambda_z     0.0967
Tmin        97.5
Cavg        66.7768
Fluctuation 77.9253

Kindly tell me, whether my results are correct or not?

Thanks and Regards,

Naresh Shirsath
 
Helmut
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2010-01-13 16:07
(5578 d 23:48 ago)

@ yes_naresh
Posting: # 4597
Views: 6,786
 

 Steady state analysis (WinNonlin)

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Dear Naresh!

❝ I have read User's Guide (WinNonlin5.2) but not get required answer.


Answer to which question? Why didn't you contact Pharsight's support?

❝ Kindly tell me, whether my results are correct or not?


Yes, they are. A note for Phoenix-Users: Cmax is not included in the estimation of lambdaz by default (on the contrary to previous versions of WinNonlin), therefore:
  Lambda_z     0.0953

Just out of curiosity: why did you sample beyond the last dosing interval (108 h)?
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