MRM values in LCMS [Bioanalytics]
Dear all
This is regarding the parameters Q1 and Q3 mass (amu). I would like to know how much variation is acceptable from the set value. For example if Q1 is found to be 413.2C for a molecule during method validation, can it vary between 412.7 to 413.7C during subject analysis. (+5).
Pl also comment if any other parameters (like CUR, CAD, IS, TEM etc) needs to be controlled as part of MRM settings.
Regards
Krishnamurthy
This is regarding the parameters Q1 and Q3 mass (amu). I would like to know how much variation is acceptable from the set value. For example if Q1 is found to be 413.2C for a molecule during method validation, can it vary between 412.7 to 413.7C during subject analysis. (+5).
Pl also comment if any other parameters (like CUR, CAD, IS, TEM etc) needs to be controlled as part of MRM settings.
Regards
Krishnamurthy
Complete thread:
- MRM values in LCMSkrishnamurthy ca 2007-04-21 11:40
- MRM values in LCMS Helmut 2007-04-22 21:01