Bioequivalence and Bioavailability Forum • clopidogrel

clopidogrel [Bioanalytics]

posted by Ohlbe – France, 2010-10-05 02:06 (5725 d 22:26 ago) – Posting: # 5994
Views: 9,563

Dear Ioanam,

In case you are still working on this topic: there is a very nice paper in press in the Journal of Chromatography B on clopidogrel back-conversion.

Development and Validation of an HPLC-MS/MS Method to Determine Clopidogrel in Human Plasma; Use of Incurred Samples to Test Back-Conversion
Luigi Silvestro, Mihaela Cristina Gheorghe, Isabela Tarcomnicu, Silviu Savu, Simona Rizea Savu, Adriana Iordachescu and Constanta Dulea
doi:10.1016/j.jchromb.2010.09.022

❝ I have also other question.

❝ There are any important differences between clopidogrel -d3, -d4 or -d6 used as internal standard regarding the final results?

In this paper they are using -d3, but with the ³⁷Cl isotope for the IS instead of the ³⁵Cl isotope for the analyte. This is quite smart: they were having cross-talk with -d3 when using the same Cl isotope, but no longer with the different isotope, without the need for an increase in the number of deuterium in the IS which could introduce or increase a difference in retention times between the analyte and the IS.

—
Regards
Ohlbe

Complete thread:

clopidogrel ioanam 2010-08-10 10:42 [Bioanalytics]
- clopidogrel Dr_Dan 2010-08-10 11:21
  - clopidogrel ioanam 2010-08-10 11:28
  - clopidogrel ioanam 2010-08-10 11:43
    - clopidogrel Ohlbe 2010-08-10 12:17
- clopidogrel ioanam 2010-08-10 14:13
  - clopidogrel Ohlbe 2010-08-10 14:59
    - clopidogrel ioanam 2010-08-10 15:17
      - clopidogrel Ohlbe 2010-08-10 16:12
        
        clopidogrel ioanam 2010-08-10 18:34
        
        clopidogrel Dr_Dan 2010-08-11 09:24
        
        clopidogrel ioanam 2010-08-11 11:08
        
        clopidogrelOhlbe 2010-10-05 00:06