Bioequivalence and Bioavailability Forum • Wagner-Nelson, Loo-Riegelman

Wagner-Nelson, Loo-Riegelman [Dissolution / BCS / IVIVC]

posted by Helmut – Vienna, Austria, 2007-03-16 16:39 (6034 d 09:03 ago) – Posting: # 583
Views: 38,498

Dear Dipesh!

First of all get the two ‘classical’ papers;
for the 1-compartment model,^[1] and for the 2-compartment model.^[2]

❝ Please suggest me the complete procedure with formula…

Below is a simple example of the Wagner-Nelson method.

Estimate k_el from t=15 to t=32: 0.1271
Calculate the partial AUCs (column 3)
Calculate amount excreted / central volume of distribution:
column 4 = column 3 x k_el
Calculate amount absorbed / central volume of distribution:
column 5 = column 2 + column 4
Calculate %absorbed:
column 6 = column 5 / asymptotic plateau (estimated as mean from t=15 to t=32: 9.976)


┌────┬──────┬───────┬───────┬───────┬──────┐

│ c1 │  c2  │   c3  │   c4  │   c5  │  c6  │

├────┼──────┼───────┼───────┼───────┼──────┤

│  t │ C(t) │ AUC(t)│ Ae/Vp │ Aa/Vp │ %abs │

├────┼──────┼───────┼───────┼───────┼──────┤

│  0 │  –   │   –   │   –   │   –   │ 0.00 │

│  1 │ 2.28 │  1.14 │  0.14 │  2.42 │ 0.24 │

│  2 │ 3.69 │  4.13 │  0.52 │  4.21 │ 0.42 │

│  3 │ 5.52 │  8.73 │  1.11 │  6.63 │ 0.66 │

│  4 │ 5.52 │ 14.25 │  1.81 │  7.33 │ 0.73 │

│  5 │ 5.08 │ 19.55 │  2.49 │  7.57 │ 0.76 │

│  6 │ 4.91 │ 24.55 │  3.12 │  8.03 │ 0.80 │

│  8 │ 4.10 │ 33.56 │  4.27 │  8.37 │ 0.84 │

│ 10 │ 3.38 │ 41.04 │  5.22 │  8.60 │ 0.86 │

│ 12 │ 3.33 │ 47.75 │  6.07 │  9.40 │ 0.94 │

│ 15 │ 2.66 │ 56.73 │  7.21 │  9.87 │ 0.99 │

│ 24 │ 0.80 │ 72.30 │  9.19 │  9.99 │ 1.00 │

│ 28 │ 0.49 │ 74.88 │  9.52 │ 10.01 │ 1.00 │

│ 32 │ 0.31 │ 76.48 │  9.72 │ 10.03 │ 1.01 │

└────┼──────┼───────┴───────┼───────┼──────┘

     │0.1271│               │  9.976│

     └──────┘               └───────┘

❝ … or some easily accessible reference on net.

A nice website is run by David Bourne.
If you want to examine your own data you will need the Java-plugin for your browser; for examples on absorption see this one of David's pages and followings.

Wagner JG, Nelson E. Per Cent Absorbed Time Plots Derived from Blood Level and / or Urinary Excretion Data. J Pharm Sci. 1965; 52: 610–1.
Loo JCK, Riegelman S. New Method for Calculating the Intrinsic Absorption Rate of Drugs. J Pharm Sci. 1968; 57: 918–28.

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Complete thread:

IVIVC Studies MGR 2007-02-02 10:10
- IVIVC Studies Helmut 2007-02-07 19:56
  - IVIVC Studies venu 2007-02-23 06:58
    - IVIVC Studies Dipesh Jayswal 2007-03-16 07:53
      - Wagner-Nelson, Loo-RiegelmanHelmut 2007-03-16 15:39
        
        Wagner-Nelson, Loo-Riegelman Dipesh Jayswal 2007-03-19 10:04
        Deconvolution drcampos 2007-03-19 15:26
        
        Numerical Deconvolution Helmut 2007-03-19 16:20
        
        Numerical Deconvolution drcampos 2007-03-19 21:17
        Numerical Deconvolution Rayhope 2021-10-07 14:25
        
        Daniel still active in the Forum? Helmut 2021-10-07 16:38
        
        Daniel still active in the Forum? Rayhope 2021-10-08 09:07
        
        Daniel still active in the Forum? Rayhope 2021-11-11 08:35
        
        Wagner-Nelson Dipesh Jayswal 2007-03-21 08:21
        
        Wagner-Nelson Helmut 2007-03-21 12:13
        
        IVIVC jesse08 2007-09-01 01:38
        
        IVIVC / Mapping Helmut 2007-09-01 14:49
        
        IVIVC / Mapping jesse08 2007-09-02 00:32
        
        IVIVC / Software Helmut 2007-09-02 12:03
        
        IVIVC / Software jesse08 2007-10-03 02:13
        
        IVIVC / Software Nirali 2007-11-21 12:38
        
        at least 3 formulations for IVIVC Helmut 2007-11-21 15:25
        
        at least 3 formulations for IVIVC Nirali 2007-11-22 07:53
        
        at least 1 formulation for Level A Helmut 2007-11-22 09:53
        
        at least 1 formulation for Level A Nirali 2007-11-27 06:05
        
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        IVIVC set up/ requirements sonu 2009-09-23 08:47