Better integration? Example [Bioanalytics]
Hi HS,
interesting stuff - I'd love to be a programmer making new ways to deal with this.
I find your example quite informative, thanks for posting it.
Let me ask a broad question here, in relation to one of your points:
It is clear that in this case the Tangential Method (TM) gives a relatively large error on a peak estimate. However, for BE purposes this might or might not be a worry since we are interested in T/R. T and R could in principle be "a lot wrong" as long as we have some trust in T/R itself; this is implicitly how it works for e.g. AUC with the noncomp. model, typically with over-estimated area before the top and under-estimated thereafter. We just pray that the error we introduce on AUC(T) is percentually the same as on AUC(R).
When the true T/R is not 1 one could argue that there could (I am not saying there is) be an issue, regardless of whether we talk chromatograms or AUCs.
I am not aware of this issue being discussed anywhere in the scientific literature, and the bioanalysts I have asked about it also don't have any information.
From your example, I would think that above all that the problem with TM is likely to be bigger if we use it in studies to characterise for example the half-life of a new chemical entity or similar.
Let me hear your or anyone elses thoughts, please.
Finally, I solemnly promise I will not make a lengthy post ever again until next time.
interesting stuff - I'd love to be a programmer making new ways to deal with this.
I find your example quite informative, thanks for posting it.
Let me ask a broad question here, in relation to one of your points:
❝ But it’s clear, that the tangential integration performs worst and should be avoided.
It is clear that in this case the Tangential Method (TM) gives a relatively large error on a peak estimate. However, for BE purposes this might or might not be a worry since we are interested in T/R. T and R could in principle be "a lot wrong" as long as we have some trust in T/R itself; this is implicitly how it works for e.g. AUC with the noncomp. model, typically with over-estimated area before the top and under-estimated thereafter. We just pray that the error we introduce on AUC(T) is percentually the same as on AUC(R).
When the true T/R is not 1 one could argue that there could (I am not saying there is) be an issue, regardless of whether we talk chromatograms or AUCs.
I am not aware of this issue being discussed anywhere in the scientific literature, and the bioanalysts I have asked about it also don't have any information.
From your example, I would think that above all that the problem with TM is likely to be bigger if we use it in studies to characterise for example the half-life of a new chemical entity or similar.
Let me hear your or anyone elses thoughts, please.
Finally, I solemnly promise I will not make a lengthy post ever again until next time.
—
Pass or fail!
ElMaestro
Pass or fail!
ElMaestro
Complete thread:
- Manual integration sagark 2010-07-29 02:26 [Bioanalytics]
- Manual integration Helmut 2010-07-29 15:30
- Manual integration ElMaestro 2010-07-29 21:07
- History Helmut 2010-07-30 01:30
- LSB - evil, terrible and annoying ElMaestro 2010-07-30 23:46
- Manual integration ElMaestro 2010-07-29 21:07
- Bad integration: Example Helmut 2010-07-30 20:24
- Bad integration: Example sagark 2010-07-31 12:57
- Bad integration: Example Helmut 2010-08-01 02:09
- Better integration? Example Helmut 2010-08-12 20:11
- Better integration? ExampleElMaestro 2010-08-12 23:14
- Better algorithms / more awareness of analysts Helmut 2010-08-13 13:43
- Better integration? ExampleElMaestro 2010-08-12 23:14
- Better integration? Example Helmut 2010-08-12 20:11
- Bad integration: Example Helmut 2010-08-01 02:09
- Bad integration: Example sagark 2010-07-31 12:57
- Manual integration keshav khude 2010-08-13 09:11
- Manual integration Helmut 2010-07-29 15:30