Initial tube or duplicate ? [Bioanalytics]

posted by Ohlbe – France, 2010-06-05 02:23 (5046 d 12:50 ago) – Posting: # 5437
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Dear Suja,

No official recommendation that I know of. The problem with using the same replicate as for the initial analysis is the extra freeze/thaw cycle. Even if during your validation you have shown that your freeze/thaw stability is within acceptable limits it does not mean that there is no effect at all. By using the duplicate sample you eliminate this possible source of variability.

Regards
Ohlbe

Regards
Ohlbe

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