Initial tube or duplicate ? [Bioanalytics]
Dear Suja,
No official recommendation that I know of. The problem with using the same replicate as for the initial analysis is the extra freeze/thaw cycle. Even if during your validation you have shown that your freeze/thaw stability is within acceptable limits it does not mean that there is no effect at all. By using the duplicate sample you eliminate this possible source of variability.
Regards
Ohlbe
No official recommendation that I know of. The problem with using the same replicate as for the initial analysis is the extra freeze/thaw cycle. Even if during your validation you have shown that your freeze/thaw stability is within acceptable limits it does not mean that there is no effect at all. By using the duplicate sample you eliminate this possible source of variability.
Regards
Ohlbe
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Regards
Ohlbe
Regards
Ohlbe
Complete thread:
- Confirmatory reanalysis of Incurred Samples shujak1 2010-06-04 10:17 [Bioanalytics]
- Initial tube or duplicate ?Ohlbe 2010-06-05 00:23
- Initial tube or duplicate ? shujak1 2010-06-08 06:05
- Initial tube or duplicate ? Dr_Dan 2010-06-08 12:08
- Initial tube or duplicate ? Ohlbe 2010-06-08 18:35
- Initial tube or duplicate ? shujak1 2010-06-09 06:06
- Initial tube or duplicate ? Dr_Dan 2010-06-09 11:30
- Initial tube or duplicate ? shujak1 2010-06-11 06:19
- Some desultory thoughts Helmut 2010-06-11 15:11
- Initial tube or duplicate ? shujak1 2010-06-11 06:19
- Initial tube or duplicate ? Dr_Dan 2010-06-09 11:30
- Initial tube or duplicate ? shujak1 2010-06-09 06:06
- Initial tube or duplicate ? Ohlbe 2010-06-08 18:35
- Initial tube or duplicate ? Dr_Dan 2010-06-08 12:08
- Initial tube or duplicate ? shujak1 2010-06-08 06:05
- Confirmatory reanalysis of Incurred Samples tusshar_adi 2010-06-24 18:55
- Confirmatory reanalysis of Incurred Samples Jagdish Bairagi 2010-07-27 16:02
- Confirmatory reanalysis of Incurred Samples Helmut 2010-07-27 16:16
- Initial tube or duplicate ?Ohlbe 2010-06-05 00:23