Recovery vs. overall efficiency [Bioanalytics]
Dear Rajashekhar,
Please read (and take into consideration !) Helmut's comments at the bottom of your post, if you want to continue this discussion !
I can't say - I'm not sure what you call absolute recovery...
Conclusion on what ? What are you trying to compare (overall efficiency vs. LLOQ
) ? What will you be using your method for ? What LLOQ will you need ? No need to go to 0.1 ng/ml if the lowest concentration you expect in your study is 5 ng/ml...
Regards
Ohlbe
Please read (and take into consideration !) Helmut's comments at the bottom of your post, if you want to continue this discussion !
❝ Please tell me the overall efficiency and absolute recovery are the same or not!
I can't say - I'm not sure what you call absolute recovery...
❝ And now, you please give me a conclusion for the following comparison i.e.
❝ Overall efficiency Vs Limit of Quantitation (here I included the LOQ and
❝ the instrumentation)
Conclusion on what ? What are you trying to compare (overall efficiency vs. LLOQ
) ? What will you be using your method for ? What LLOQ will you need ? No need to go to 0.1 ng/ml if the lowest concentration you expect in your study is 5 ng/ml...Regards
Ohlbe
Complete thread:
- IS (polarity switching mode) drajasekhar 2009-12-27 10:43 [Bioanalytics]
- IS (polarity switching mode) Ohlbe 2009-12-28 01:00
- LC-MS/MS method in the ionization Switching modes drajasekhar 2009-12-28 06:32
- Recovery? Ohlbe 2009-12-30 13:51
- Recovery? drajasekhar 2009-12-30 14:45
- Recovery vs. overall efficiency Ohlbe 2009-12-30 17:36
- Recovery vs. overall efficiency drajasekhar 2009-12-30 18:17
- Recovery vs. overall efficiencyOhlbe 2009-12-30 19:16
- Recovery vs. overall efficiency drajasekhar 2009-12-30 19:50
- Recovery vs. overall efficiency Helmut 2009-12-30 19:58
- Recovery vs. overall efficiency drajasekhar 2009-12-30 20:35
- Recovery vs. overall efficiency Helmut 2009-12-30 20:38
- Recovery vs. overall efficiency Ohlbe 2009-12-30 20:43
- Recovery vs. overall efficiency drajasekhar 2009-12-30 20:54
- Recovery vs. overall efficiency drajasekhar 2009-12-30 20:35
- Recovery vs. overall efficiency drajasekhar 2009-12-30 18:17
- Recovery vs. overall efficiency Ohlbe 2009-12-30 17:36
- Recovery? drajasekhar 2009-12-30 14:45
- Recovery? Ohlbe 2009-12-30 13:51
- Matrix Effect drajasekhar 2009-12-28 07:00
- Matrix Effect Helmut 2009-12-28 14:18
- Matrix Effect Ohlbe 2009-12-30 13:53
- LC-MS/MS method in the ionization Switching modes drajasekhar 2009-12-28 06:32
- IS (polarity switching mode) Ohlbe 2009-12-28 01:00
