Bioequivalence and Bioavailability Forum • IS (polarity switching mode)

IS (polarity switching mode) [Bioanalytics]

posted by drajasekhar – India, 2009-12-27 11:43 (6026 d 21:22 ago) – Posting: # 4524
Views: 7,879
(edited on 2009-12-27 12:09)

Dear Members,

This is with regard to use of internal standards in LCMSMS bioanalytical techniques especially in polarity switching mode. For the determination of two drugs (one showing good response at positive ion mode and other at negative ion mode) in human plasma by LC-MS-MS; for this how many internal standards we should use? Using one internal standard for both the polarities (positive or negative) is it legitimate?

Thanks in Advance

Rajasekhar

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Edit: Subject line changed. [Jaime]

Complete thread:

IS (polarity switching mode)drajasekhar 2009-12-27 10:43 [Bioanalytics]
- IS (polarity switching mode) Ohlbe 2009-12-28 01:00
  - LC-MS/MS method in the ionization Switching modes drajasekhar 2009-12-28 06:32
    - Recovery? Ohlbe 2009-12-30 13:51
      - Recovery? drajasekhar 2009-12-30 14:45
        
        Recovery vs. overall efficiency Ohlbe 2009-12-30 17:36
        
        Recovery vs. overall efficiency drajasekhar 2009-12-30 18:17
        
        Recovery vs. overall efficiency Ohlbe 2009-12-30 19:16
        Recovery vs. overall efficiency drajasekhar 2009-12-30 19:50
        Recovery vs. overall efficiency Helmut 2009-12-30 19:58
        
        Recovery vs. overall efficiency drajasekhar 2009-12-30 20:35
        
        Recovery vs. overall efficiency Helmut 2009-12-30 20:38
        Recovery vs. overall efficiency Ohlbe 2009-12-30 20:43
        
        Recovery vs. overall efficiency drajasekhar 2009-12-30 20:54
  - Matrix Effect drajasekhar 2009-12-28 07:00
    - Matrix Effect Helmut 2009-12-28 14:18
    - Matrix Effect Ohlbe 2009-12-30 13:53