sample centrifugation [Study Per­for­mance]

posted by Jaime_R – Barcelona, 2006-10-12 15:15 (7199 d 04:13 ago) – Posting: # 315
Views: 10,109

Hi ssk!

❝ What does the g stand for.


g: approximately 9.81 m/s².
It's good practice to state something like '1500 × g' (1500times acceleration due to earth's gravity) in order to be able to compare different centrifuges.
Stating rpm alone means nothing (since the diameter of the rotor is not given).

For a conversion have a look at this document or this nomogram.

❝ What is the standard temperature at which the centrifugation usually done.


Cooled ;-)
Actually you don't know the temperature of the sample, but it's quite common to set the temperature of the centrifuge to +4 °C.

❝ How are these parameters calculated by the sponsor (rpm,temperature and the duration).


As already stated it should allow for easy separation of plasma.
I don't believe that sponsors do calculate anything - mainly they are skillful in 'copy-and-paste'... ;-)

Regards, Jaime

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