Bioequivalence and Bioavailability Forum

A dumb answer [Bioanalytics]

posted by jag009  – NJ, 2024-08-12 16:35 (35 d 15:40 ago) – Posting: # 24148
Views: 1,119

Hi Helmut,

❝ ❝ A dumb question …

❝ You know that there are no dump questions.

Just a way to catch your attention

❝ Are you talking about a drug with protein binding? That’s easy. Protein binding is caused by Van der Waals force, which is extremely weak. In a nutshell, in any extraction (liquid-liquid, solid-phase), or protein-precipitation the protein-drug bonds will be practically imme­dia­tely cleaved and therefore, from the extract you get always the total concentration.

Yes, according to the text it was protein-precipitation extraction from plasma during sample pre-treatment phase. It's a drug w high protein binding. So it's total drug?

Thx
J

Complete thread:

• A dumb question jag009 2024-08-12 13:17 [Bioanalytics] 
  • A dumb answer Helmut 2024-08-12 13:45
    • A dumb answerjag009 2024-08-12 14:35
      • A dumb answer Helmut 2024-08-12 15:01
        • A dumb answer ElMaestro 2024-08-12 15:13
          • A dumb answer Helmut 2024-08-12 15:30
  • An alternate dumb answer Ohlbe 2024-08-12 21:37
    • An alternate dumb answer jag009 2024-08-13 06:27
    • Unconjugated ≠ free Helmut 2024-08-13 07:31
      • Unconjugated ≠ free jag009 2024-08-26 16:19
  • A dumb question Achievwin 2024-08-27 03:02