A dumb answer [Bioanalytics]

posted by Helmut Homepage – Vienna, Austria, 2024-08-12 15:45 (331 d 18:10 ago) – Posting: # 24147
Views: 4,317

Hi John,

❝ A dumb question …

You know that there are no dump questions.

❝ Is there a way to find out whether the total drug concentration is measured from a bioanalytical report (no method development report, just bioanalytical and validation reports) if there is no sentence/discussion about that aspect? I read through a report that I was given and I didn't find info on this.

More information, please.
Are you talking about a drug with protein binding? That’s easy. Protein binding is caused by Van der Waals force, which is extremely weak. In a nutshell, in any extraction (liquid-liquid, solid-phase), or protein-precipitation the protein-drug bonds will be practically imme­dia­tely cleaved and therefore, from the extract you get always the total concentration.
If you are interested in the free concentration, you would have to use ultra-filtration or -centrifugation of the same sample, measure this concentration and the total. The protein binding: $$\small{PB(\%)=100\frac{C_\text{total}-C_\text{free}}{C_\text{total}}}$$ Important for new drugs but completely uninteresting in BE.

Liposome encapsulated drugs and their relatives are different beasts.

Dif-tor heh smusma 🖖🏼 Довге життя Україна! [image]
Helmut Schütz
[image]

The quality of responses received is directly proportional to the quality of the question asked. 🚮
Science Quotes

Complete thread:

UA Flag
Activity
 Admin contact
23,428 posts in 4,929 threads, 1,684 registered users;
63 visitors (0 registered, 63 guests [including 20 identified bots]).
Forum time: 09:56 CEST (Europe/Vienna)

To know that we know what we know,
and to know that we do not know what we do not know,
that is true knowledge.    Nicolaus Copernicus

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5