dead / void volume [Bioanalytics]

posted by Helmut Homepage – Vienna, Austria, 2021-10-03 21:14 (20 d 08:13 ago) – Posting: # 22617
Views: 248

Hi Bioanalytical,

» For a bio analytical method how relevant is the evaluation of dead volume or void volume

Interesting1 but not relevant as long the method passes all criteria in method validation.

» For a method having very short retention time within void volume what should be the approach

All the usual stuff: Pumps and mixing systems with low volumes, low injection volume, narrow bore tubings as short as possible, correct column nuts and ferrules, clean frits, no dead volume on top of aged column(s)…

» Is it ok and should the evaluation of dead / void volume be ignored as it is estimated by LCMSMS technique

See above and this goody.2


  1. Hint: Thiourea SC(NH2)2 has practically no retention on reverse phase and can be used to estimate t0 of the entire system (including the column).
  2. Dolan JW. Extracolumn Effects. LCGC North America. 2008; 26(11): 1092–8. [image] Open access.

Dif-tor heh smusma 🖖
Helmut Schütz
[image]

The quality of responses received is directly proportional to the quality of the question asked. 🚮
Science Quotes

Complete thread:

Activity
 Admin contact
21,753 posts in 4,548 threads, 1,544 registered users;
online 4 (0 registered, 4 guests [including 4 identified bots]).
Forum time: Sunday 05:28 CEST (Europe/Vienna)

They were “so intent of making everything numerical”
that they frequently missed seeing
what was there to be seen.    Barbara McClintock

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5