More on IS response variations [Regulatives / Guidelines]

posted by ElMaestro  – Denmark, 2019-11-12 13:38 (757 d 03:03 ago) – Posting: # 20790
Views: 3,417

Hi Hötzi,

» I don’t see how that can affect the IS (and analyte) response unless someone works with peak heights (who does it nowadays?)…

As a general rule the S:N become more unfavourable as the column ages and peak separation suffers. This affects the identifcation of peaks and their integration. It will be more pronounced for the analyte near the LLOQ than for the IS.

Pass or fail!
ElMaestro

Complete thread:

Activity
 Admin contact
21,788 posts in 4,557 threads, 1,548 registered users;
online 15 (0 registered, 15 guests [including 3 identified bots]).
Forum time: Wednesday 16:41 CET (Europe/Vienna)

There is no adequate defense, except stupidity,
against the impact of a new idea.    Percy Williams Bridgman

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5