More on IS response variations [Regulatives / Guidelines]

posted by ElMaestro  – Belgium?, 2019-11-12 12:38  – Posting: # 20790
Views: 1,063

Hi Hötzi,

» I don’t see how that can affect the IS (and analyte) response unless someone works with peak heights (who does it nowadays?)…

As a general rule the S:N become more unfavourable as the column ages and peak separation suffers. This affects the identifcation of peaks and their integration. It will be more pronounced for the analyte near the LLOQ than for the IS.

I could be wrong, but...
Best regards,
ElMaestro

Complete thread:

Activity
 Admin contact
20,101 posts in 4,243 threads, 1,382 registered users;
online 11 (0 registered, 11 guests [including 6 identified bots]).
Forum time (Europe/Vienna): 20:33 UTC

Trust, but verify.    Anonymous (Russian proverb)

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5