Bioequivalence and Bioavailability Forum • More on IS response variations

More on IS response variations [Regulatives / Guidelines]

posted by ElMaestro – Denmark, 2019-11-12 14:38 (1986 d 11:42 ago) – Posting: # 20790
Views: 6,119

Hi Hötzi,

❝ I don’t see how that can affect the IS (and analyte) response unless someone works with peak heights (who does it nowadays?)…

As a general rule the S:N become more unfavourable as the column ages and peak separation suffers. This affects the identifcation of peaks and their integration. It will be more pronounced for the analyte near the LLOQ than for the IS.

—
Pass or fail!
ElMaestro

Complete thread:

IS response variations Ohlbe 2019-09-04 19:03 [Regulatives / Guidelines]
- IS response variations nobody 2019-09-06 16:21
- More on IS response variations Ohlbe 2019-11-12 11:32
  - More on IS response variations ElMaestro 2019-11-12 12:27
    - More on IS response variations Helmut 2019-11-12 13:24
      - More on IS response variationsElMaestro 2019-11-12 13:38
        
        More on IS response variations Helmut 2019-11-12 13:41
      - More on IS response variations Ohlbe 2019-11-12 13:56
        
        More on IS response variations ElMaestro 2019-11-12 16:25
        
        More on IS response variations Ohlbe 2019-11-12 18:14
        
        More on IS response variations ElMaestro 2019-11-12 18:39
        
        Not more on IS response variations Ohlbe 2019-11-13 00:06
        
        Not more on IS response variations nobody 2019-11-13 10:06
        
        the run passes = there is no problem? ElMaestro 2019-11-13 17:35
        
        the run passes = there is no problem? nobody 2019-11-13 19:17