Evaluation of Solution stability (with post extracted matrix based samples) [Bioanalytics]

posted by Ravuri sindhura – India, 2019-11-07 12:17  – Posting: # 20760
Views: 1,774

Hi,

Here with I had a query with respect to solution stability in Bio analysis method validation.
General approach of evaluating Solution stability is acquiring/analyzing samples which are prepared in organic solvents/Aqueous after dilution from master stock.

But During evaluation of solution stability for one molecule, we faced issue with poor chromatography (peak shape issues).

we observed Poor chromatography after acquisition of few aqueous/organic samples.

Is it acceptable to adopt an alternate approach for evaluation of stock solution stability, where samples are analyzed after spiking to post extraction samples (blank sample processed and spiked with required concentration)?

Regards
Sindhura

Complete thread:

Activity
 Admin contact
20,641 posts in 4,327 threads, 1,437 registered users;
online 15 (1 registered, 14 guests [including 9 identified bots]).
Forum time: 10:40 CEST (Europe/Vienna)

I have had my results for a long time:
but I do not yet know how I am to arrive
at them.    Carl Friedrich Gauß

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5