Evaluation of Solution stability (with post extracted matrix based samples) [Bioanalytics]

posted by Ravuri sindhura – India, 2019-11-07 12:17 (436 d 03:02 ago) – Posting: # 20760
Views: 2,192

Hi,

Here with I had a query with respect to solution stability in Bio analysis method validation.
General approach of evaluating Solution stability is acquiring/analyzing samples which are prepared in organic solvents/Aqueous after dilution from master stock.

But During evaluation of solution stability for one molecule, we faced issue with poor chromatography (peak shape issues).

we observed Poor chromatography after acquisition of few aqueous/organic samples.

Is it acceptable to adopt an alternate approach for evaluation of stock solution stability, where samples are analyzed after spiking to post extraction samples (blank sample processed and spiked with required concentration)?

Regards
Sindhura

Complete thread:

Activity
 Admin contact
21,303 posts in 4,441 threads, 1,487 registered users;
online 2 (0 registered, 2 guests [including 2 identified bots]).
Forum time: Saturday 15:19 CET (Europe/Vienna)

If you can’t solve a problem, then there is an easier problem
you can solve: find it.    George Pólya

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5