Free and total analyte [Bioanalytics]

posted by Ladi – Thailand, 2019-07-29 13:15 (536 d 07:20 ago) – Posting: # 20463
Views: 2,172

Hi Mithu,

» » Are you referring by “free” to the fraction which is not protein-bound?
» Yes.
» Here i am talking about Scopolamine Patch 1/72 hrs. The label is talking about free as well as total Scopolamine (free + conjugated analyte).

My understanding with conjugated analyte in this case is Scopolamine + 'a moiety' such as glucuronide moiety. Scopolamine dose have sulfate conjugated and glucuronide conjugated forms as its metabolites. So in this case free Scopolamine is the measurement of unconjugated Scopolamine only while total Scopolamine is the measurement of free Scopolamine + deconjugated Scopolamine (after some hydrolysis reactions). Similar case with Ezetimibe. I think nothing have to do with protein binding here.

This journal talks about the free and conjugated Scopolamine>>

Making sense?

Complete thread:

 Admin contact
21,303 posts in 4,441 threads, 1,488 registered users;
online 6 (0 registered, 6 guests [including 5 identified bots]).
Forum time: Friday 19:36 CET (Europe/Vienna)

Statistics is the grammar of science.    Karl Pearson

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz