Bioequivalence and Bioavailability Forum

Hi Mithu,

❝ ❝ Are you referring by “free” to the fraction which is not protein-bound?

❝

❝ Yes.

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❝ Here i am talking about Scopolamine Patch 1/72 hrs. The label is talking about free as well as total Scopolamine (free + conjugated analyte).

My understanding with conjugated analyte in this case is Scopolamine + 'a moiety' such as glucuronide moiety. Scopolamine dose have sulfate conjugated and glucuronide conjugated forms as its metabolites. So in this case free Scopolamine is the measurement of unconjugated Scopolamine only while total Scopolamine is the measurement of free Scopolamine + deconjugated Scopolamine (after some hydrolysis reactions). Similar case with Ezetimibe. I think nothing have to do with protein binding here.

This journal talks about the free and conjugated Scopolamine>> https://www.ncbi.nlm.nih.gov/pubmed/18970269


Making sense?
Ladi