LLOQ ≤5% Cmax [Bioanalytics]

posted by Developper bioanalyste  – Algeria, 2019-05-02 11:52 (672 d 04:28 ago) – Posting: # 20266
Views: 2,402

Think you for your explanations, for the first question i meant that actually i calculate my LIQ basing on formula of 5% of lower Cmax observed in bibliography i try to go lower than that on my HPLC to cover residual concentrations if observed later, we haven't yet a pharmacologue in our CRO (we are just team of scientists working on amoxicilline bioéquivalence), we have been taught that there is a formul to calculate LIQ from AUC ?

2/My question about concentration of internal standard: actually i use cefadroxil as described in articles as IS most recomended, in articles they describe different concentrations, i nedd to know how can we fix exactly this concentration , is it in relation with analyte concentration wich is amoxicilline at ULOQ or i have to test different concentrations of IS then analyte/IS later confused: ?



i hope receiving responses frome other member of forum, i dont know HOW !

Complete thread:

Activity
 Admin contact
21,363 posts in 4,462 threads, 1,492 registered users;
online 13 (1 registered, 12 guests [including 2 identified bots]).
Forum time: Thursday 16:21 UTC (Europe/Vienna)

Being really good at C++ is like being really good
at using rocks to sharpen sticks.    Thant Tessman

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5