LLOQ ≤5% Cmax [Bioanalytics]

posted by Developper bioanalyste  – Algeria, 2019-05-02 13:52  – Posting: # 20266
Views: 717

Think you for your explanations, for the first question i meant that actually i calculate my LIQ basing on formula of 5% of lower Cmax observed in bibliography i try to go lower than that on my HPLC to cover residual concentrations if observed later, we haven't yet a pharmacologue in our CRO (we are just team of scientists working on amoxicilline bioéquivalence), we have been taught that there is a formul to calculate LIQ from AUC ?

2/My question about concentration of internal standard: actually i use cefadroxil as described in articles as IS most recomended, in articles they describe different concentrations, i nedd to know how can we fix exactly this concentration , is it in relation with analyte concentration wich is amoxicilline at ULOQ or i have to test different concentrations of IS then analyte/IS later confused: ?



i hope receiving responses frome other member of forum, i dont know HOW !

Complete thread:

Activity
 Mix view
Bioequivalence and Bioavailability Forum |  Admin contact
19,694 posts in 4,181 threads, 1,355 registered users;
online 9 (0 registered, 9 guests [including 8 identified bots]).
Forum time (Europe/Vienna): 14:23 CEST

A central lesson of science is that to understand complex issues
(or even simple ones), we must try to free our minds of dogma and
to guarantee the freedom to publish, to contradict, and to experiment.
Arguments from authority are unacceptable.    Carl Sagan

The BIOEQUIVALENCE / BIOAVAILABILITY FORUM is hosted by
BEBAC Ing. Helmut Schütz
HTML5