ASS stabilization [Bioanalytics]
Hi Norah,
In my CRO we had two methods (anticoagulant K3EDTA or Li-heparin). Example for 10 mL vacutainers.
Handling & storage
❝ I'm preparing a BE protocol regarding, enteric delaying release aspirin […] Could you please tell me what are you recommendations to avoid the degradation of active ingredient in metabolite.
In my CRO we had two methods (anticoagulant K3EDTA or Li-heparin). Example for 10 mL vacutainers.
- Stabilizer H3PO4
Prepare 5% orthophosporic acid (59 mL 85% o-H3PO4 p.a. diluted to 1000 mL).
Caution: Even diluted phosphoric acid is strongly acidic; wear gloves and eye protection.
- Stabilizer potassium fluoride
Prepare cold saturated KF solution (100 g KF p.a. in 100 ml water).
Caution: KF is poisonous. Observe the usual rules.
Handling & storage
Put blood samples immediately after draw in an ice-bath. Allow 10 minutes for equilibration and centrifuge at ~2000×g at +4 ℃ for 20 minutes.
- Mix plasma with 5% H3PO4 1:1 (exactly!). Store at ≤20 ℃.
- Store at ≤70 ℃.
—
Dif-tor heh smusma 🖖🏼 Довге життя Україна!![[image]](https://static.bebac.at/pics/Blue_and_yellow_ribbon_UA.png)
Helmut Schütz
![[image]](https://static.bebac.at/img/CC by.png)
The quality of responses received is directly proportional to the quality of the question asked. 🚮
Science Quotes
Dif-tor heh smusma 🖖🏼 Довге життя Україна!
![[image]](https://static.bebac.at/pics/Blue_and_yellow_ribbon_UA.png)
Helmut Schütz
![[image]](https://static.bebac.at/img/CC by.png)
The quality of responses received is directly proportional to the quality of the question asked. 🚮
Science Quotes
Complete thread:
- aspirin degradation Norah 2018-12-11 11:30 [Bioanalytics]
- ASS stabilizationHelmut 2019-02-02 16:56