## No random calibrators (and QCs) [Bioanalytics]

Hi Bert,

» Is there any guidance for the location of calibration samples in an analytical run?

Not that I recall. I agree with ElMaestro’s comments.

» Should this be at-random?

Not a good idea – even if there is no substantial carry-over (see the EMA’s GL Section 4.2.2). If you decide to inject calibrators at the start and end of the batch do so with increasing concentrations which minimizes the relative impact of carry-over. If you have calibrators within the set of unknown samples inject a blank after. The same is applicable for QC samples as well.

» (Like it, called Junior )

Not referring to your age or experience but the number of posts in the forum.
Junior : <25 Regular: 25 < 100 Senior : 100 < 500 Hero   : >500

Dif-tor heh smusma 🖖
Helmut Schütz

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