No random calibrators (and QCs) [Bioanalytics]

posted by Helmut Homepage – Vienna, Austria, 2019-02-02 15:49 (805 d 09:47 ago) – Posting: # 19846
Views: 1,752

Hi Bert,

» Is there any guidance for the location of calibration samples in an analytical run?

Not that I recall. I agree with ElMaestro’s comments.

» Should this be at-random?

Not a good idea – even if there is no substantial carry-over (see the EMA’s GL Section 4.2.2). If you decide to inject calibrators at the start and end of the batch do so with increasing concentrations which minimizes the relative impact of carry-over. If you have calibrators within the set of unknown samples inject a blank after. The same is applicable for QC samples as well.

» (Like it, called Junior :-) )

Not referring to your age or experience but the number of posts in the forum. :-D
Junior : <25
Regular: 25 < 100
Senior : 100 < 500
Hero   : >500

Dif-tor heh smusma 🖖
Helmut Schütz

The quality of responses received is directly proportional to the quality of the question asked. 🚮
Science Quotes

Complete thread:

 Admin contact
21,419 posts in 4,475 threads, 1,511 registered users;
online 11 (0 registered, 11 guests [including 5 identified bots]).
Forum time: Sunday 02:36 CEST (Europe/Vienna)

Nothing fails like success because you do not learn anything from it.
The only thing we ever learn from is failure.
Success only confirms our superstitions.    Kenneth E. Boulding

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz