## AUCinf, MRT, Partial AUC [Software]

Dear PKBR,

» When we do NCA we have obs and pred parameters. Which is better to use? Ex: AUCobs or AUCpred?

No one else but Helmut answered that question here

» When we do plasma and microdialysate experiments, but the time collections are not the same, for example, plasma collections was performed until 9 hr and microdialysis collections in tissue until 8.5 hr. Is it possible to compare the MRT in this case with different time of experiment?

MRT is derived from AUC and AUMC.

In WNL wordings

If the coverage of PK profile is enough, the estimation of MRT would be fair enough too.

» What about AUC? For AUC would be possible to extrapolate the concentration up to 9hr considering the k?

look at the partial AUC calculation rules in WNL guide cited here. I think it is what are you looking for.

» When we calculate t1/2 and MRT after iv bolus dosing and these values are different, which could be the reasons if the k is not statistically different?

The result of statistical test depends on the assumption of distribution of parameter.

Thus since HL = ln(2)/kel

the distribution of parameters won't be the same.

Simple example in R

» When we do NCA we have obs and pred parameters. Which is better to use? Ex: AUCobs or AUCpred?

No one else but Helmut answered that question here

» When we do plasma and microdialysate experiments, but the time collections are not the same, for example, plasma collections was performed until 9 hr and microdialysis collections in tissue until 8.5 hr. Is it possible to compare the MRT in this case with different time of experiment?

MRT is derived from AUC and AUMC.

In WNL wordings

**MRTlast = AUMClast/AUClast**(bolus case)If the coverage of PK profile is enough, the estimation of MRT would be fair enough too.

» What about AUC? For AUC would be possible to extrapolate the concentration up to 9hr considering the k?

look at the partial AUC calculation rules in WNL guide cited here. I think it is what are you looking for.

» When we calculate t1/2 and MRT after iv bolus dosing and these values are different, which could be the reasons if the k is not statistically different?

The result of statistical test depends on the assumption of distribution of parameter.

Thus since HL = ln(2)/kel

the distribution of parameters won't be the same.

Simple example in R

`> t.test(1:10, y = c(7:20)) `

Welch Two Sample t-test

data: 1:10 and c(7:20)

t = -5.4349, df = 21.982, p-value = 1.855e-05

alternative hypothesis: true difference in means is not equal to 0

95 percent confidence interval:

-11.052802 -4.947198

sample estimates:

mean of x mean of y

5.5 13.5

> t.test(log(2)/1:10, y = c(log(2)/7:20))

Welch Two Sample t-test

data: log(2)/1:10 and c(log(2)/7:20)

t = 2.3964, df = 9.1376, p-value = 0.03974

alternative hypothesis: true difference in means is not equal to 0

95 percent confidence interval:

0.008506605 0.283884254

sample estimates:

mean of x mean of y

0.20302061 0.05682518

—

Kind regards,

Mittyri

Kind regards,

Mittyri

### Complete thread:

- Phoenix Winnonlin PKBR 2019-01-25 18:27 [Software]
- AUCinf, MRT, Partial AUCmittyri 2019-01-26 22:42
- AUCinf, MRT, Partial AUC PKBR 2019-01-28 18:54

- AUCinf, MRT, Partial AUCmittyri 2019-01-26 22:42