Interference from Deuterated IS [Bioanalytics]

posted by Ohlbe – France, 2019-01-10 22:36 (782 d 13:54 ago) – Posting: # 19769
Views: 2,423

Dear VKB2207,

» You can probably evaluate the % contribution from IS at RT of drug

Sure. It's called the intercept :-D

» and then apply this factor in CC, QC nominal concentrations.

Certainly not. It is taken into consideration when calculating the calibration curve parameters, unless you are forcing the intercept through 0 (which is not to be done in bioanalysis).

» Hence measured concentrations would be inaccurate.

Not necessarily at high level of concentrations. Could indeed be problematic closer to the LLOQ.

Regards
Ohlbe

Complete thread:

Activity
 Admin contact
21,361 posts in 4,461 threads, 1,492 registered users;
online 7 (1 registered, 6 guests [including 4 identified bots]).
Forum time: Wednesday 12:31 CET (Europe/Vienna)

The art and science of asking questions
is the source of all knowledge.    Thomas Berger

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5