Bioequivalence and Bioavailability Forum

Interference from Deuterated IS [Bioanalytics]

posted by Ohlbe – France, 2019-01-10 23:36 (1924 d 06:23 ago) – Posting: # 19769
Views: 3,368

Dear VKB2207,

❝ You can probably evaluate the % contribution from IS at RT of drug

Sure. It's called the intercept

❝ and then apply this factor in CC, QC nominal concentrations.

Certainly not. It is taken into consideration when calculating the calibration curve parameters, unless you are forcing the intercept through 0 (which is not to be done in bioanalysis).

❝ Hence measured concentrations would be inaccurate.

Not necessarily at high level of concentrations. Could indeed be problematic closer to the LLOQ.

Complete thread:

• Interference from Deuterated IS cakhatri 2019-01-10 07:20 [Bioanalytics] 
  • Interference from Deuterated IS VKB2207 2019-01-10 20:48
    • Interference from Deuterated ISOhlbe 2019-01-10 22:36
  • Interference from Deuterated IS Ohlbe 2019-01-10 23:41
    • Interference from Deuterated IS cakhatri 2019-01-11 09:28
  • Interference from Deuterated IS ElMaestro 2019-01-11 09:49