## Interference from Deuterated IS [Bioanalytics]

Dear VKB2207,

» You can probably evaluate the % contribution from IS at RT of drug

Sure. It's called the intercept

» and then apply this factor in CC, QC nominal concentrations.

Certainly not. It is taken into consideration when calculating the calibration curve parameters, unless you are forcing the intercept through 0 (which is not to be done in bioanalysis).

» Hence measured concentrations would be inaccurate.

Not necessarily at high level of concentrations. Could indeed be problematic closer to the LLOQ.

Regards
Ohlbe