Distribution to erythrocytes of Methylphenidate [Bioanalytics]
Dear EKY,
If I interpret correctly what you wrote, concentrations are comparable in plasma and erythrocytes. So hemolysis will not release massive amounts of the drug or metabolites in plasma, contrary to e.g. ciclosporine.
Yes, possibly, by other ways. If you are using LC-MS/MS, you may have differences in matrix effects in hemolysed samples, but you are supposed to check for this during method validation. If you are using HPLC/UV, you may get chromatographic interferences. Whatever the method, you may have problems with recovery, or with analyte stability (see for instance this paper).
❝ According to available data that methylphenidate and its metabolites are distributed between plasma (57%) and erythrocytes (43%) in blood.
If I interpret correctly what you wrote, concentrations are comparable in plasma and erythrocytes. So hemolysis will not release massive amounts of the drug or metabolites in plasma, contrary to e.g. ciclosporine.
❝ Can the respective plasma concentrations from hemolysis samples be fallacious?
Yes, possibly, by other ways. If you are using LC-MS/MS, you may have differences in matrix effects in hemolysed samples, but you are supposed to check for this during method validation. If you are using HPLC/UV, you may get chromatographic interferences. Whatever the method, you may have problems with recovery, or with analyte stability (see for instance this paper).
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Regards
Ohlbe
Regards
Ohlbe
Complete thread:
- Distribution to erythrocytes of Methylphenidate EKY 2019-01-04 03:08
- Distribution to erythrocytes of MethylphenidateOhlbe 2019-01-06 19:07