Distribution to erythrocytes of Methylphenidate [Bioanalytics]

posted by Ohlbe – France, 2019-01-06 19:07  – Posting: # 19736
Views: 506

Dear EKY,

» According to available data that methylphenidate and its metabolites are distributed between plasma (57%) and erythrocytes (43%) in blood.

If I interpret correctly what you wrote, concentrations are comparable in plasma and erythrocytes. So hemolysis will not release massive amounts of the drug or metabolites in plasma, contrary to e.g. ciclosporine.

» Can the respective plasma concentrations from hemolysis samples be fallacious?

Yes, possibly, by other ways. If you are using LC-MS/MS, you may have differences in matrix effects in hemolysed samples, but you are supposed to check for this during method validation. If you are using HPLC/UV, you may get chromatographic interferences. Whatever the method, you may have problems with recovery, or with analyte stability (see for instance this paper).

Regards
Ohlbe

Complete thread:

Activity
 Mix view
Bioequivalence and Bioavailability Forum |  Admin contact
19,567 posts in 4,150 threads, 1,340 registered users;
online 8 (1 registered, 7 guests [including 4 identified bots]).
Forum time (Europe/Vienna): 16:41 CEST

Mediocrity knows nothing higher than itself,
but talent instantly recognizes genius.    Arthur Conan Doyle

The BIOEQUIVALENCE / BIOAVAILABILITY FORUM is hosted by
BEBAC Ing. Helmut Schütz
HTML5