## Purpose of S:N? [Bioanalytics]

» Is that somewhere stated, that peak height is the signal?

Not only the two references I gave above but all about detection in chromatography since the 1960s.

» You need a baseline (whatever that is) to establish this signal (as for the area, so both parameters don't fall out of the clear blue sky but might be considered convention methods, as the PO has noticed). Why not using the same signal as used for calculation of P/A, i.e. most often area? OR ratio of area with IS?

Simple. Given the CDS's settings appropriate to integrate the peak (bunching, smoothing, slope limits for start/end, area threshold) the noise will not be integrated.
BTW, I'm curious how other members of the forum calculate the noise.

Dif-tor heh smusma 🖖
Helmut Schütz

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