Purpose of S:N? [Bioanalytics]

posted by nobody – 2018-11-09 12:08 (1045 d 15:36 ago) – Posting: # 19555
Views: 5,761

» » Do you evaluate peak height as the signal for your assay? Or peak area?
»
» Mainly the latter. However,

the signal
» is the signal
» is the signal

- not its integral over time. :-D

Is that somewhere stated, that peak height is the signal? You need a baseline (whatever that is) to establish this signal (as for the area, so both parameters don't fall out of the clear blue sky but might be considered convention methods, as the PO has noticed). Why not using the same signal as used for calculation of P/A, i.e. most often area? OR ratio of area with IS?

Kindest regards, nobody

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