## Yes it went a little fast [Bioanalytics]

Formally, the limit of detection (LOD) is defined by the S:N ratio. The peak you can definitely distinguish from noise (Limit of blank) with your method. In bioanalytics the LLOQ is by definition the concentration you can determine with P/A of 20%/+/-20% (by a convention method, i.e spiked samples with n=......).

If you (or anybody else in the room...) think your LOD is higher than the LLOQ, you have a problem, in deed. Otherwise... see Helllcourages statement.

Kindest regards, nobody