Bioequivalence and Bioavailability Forum

Dear giang nidqc

❝ Could it be a possibility that the absence of one APIs may affect the final concentration of other APIs (regarding sample preparation and peak response)?

I agree with Helmut's post above. There is just one thing I'm thinking of: how was the spiking of calibration and QC samples done when you used 5 analytes ? Did you use a single spiking solution containing all analytes (usual practice), or separate working solutions ? If you used a single, combined solution at each level concentration, no problem. If you used separate spiking solutions, you will have a different proportion of solvent added to the plasma if you have less analytes, which may influence your recovery and matrix effects.