Ratio area analyte vs internal standard [Bioanalytics]

posted by Ohlbe – France, 2015-08-17 20:19 (3469 d 08:35 ago) – Posting: # 15288
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Dear Joy,

❝ I couldn't find rules in EMEA or FDA guideline, related to Ratio area of analyte vs internal standard (IS) for LLOQ in calibration curve.


AFAIK there is none.

If you are using a stable isotope IS: you need to consider the isotopic purity of your IS (so basically, the signal you get in the channel of the analyte when injecting a pure solution of the IS). You may need to adapt the amount of IS you add if the purity is not high enough.

Regards
Ohlbe

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