Ratio area analyte vs internal standard [Bioanalytics]
Dear Joy,
AFAIK there is none.
If you are using a stable isotope IS: you need to consider the isotopic purity of your IS (so basically, the signal you get in the channel of the analyte when injecting a pure solution of the IS). You may need to adapt the amount of IS you add if the purity is not high enough.
❝ I couldn't find rules in EMEA or FDA guideline, related to Ratio area of analyte vs internal standard (IS) for LLOQ in calibration curve.
AFAIK there is none.
If you are using a stable isotope IS: you need to consider the isotopic purity of your IS (so basically, the signal you get in the channel of the analyte when injecting a pure solution of the IS). You may need to adapt the amount of IS you add if the purity is not high enough.
—
Regards
Ohlbe
Regards
Ohlbe
Complete thread:
- Ratio area analyte vs internal standard joy_fm 2015-08-10 08:43 [Bioanalytics]
- Ratio area analyte vs internal standardOhlbe 2015-08-17 18:19