Bioequivalence and Bioavailability Forum • Internal Standard Variation

Internal Standard Variation [Bioanalytics]

posted by Helmut – Vienna, Austria, 2007-09-09 02:37 (6853 d 17:07 ago) – Posting: # 1063
Views: 17,440

Dear Harish & Ohlbe,

now it becomes interesting!

100% agree with Ohlbe about low responses, but first we have to sort out what exactly we are meaning by ‘IS variation’.

Maybe I’m wrong, but I got the impression from Harish’s post that he was talking about varying responses of single measurements within an analytical batch.

❝ I agree with HS, the main question will be: is the peak area ratio affected by the IS variation or not ?

❝

❝ Supposing the ratio is not affected,...

Ohlbe, I’m not sure whether you are talking about duplicates?
If measuring singlets, we don’t know whether or not the ratio is affected; we only may notice an ‘unusual’ IS response.

—
Dif-tor heh smusma 🖖🏼 Довге життя Україна!
Helmut Schütz

The quality of responses received is directly proportional to the quality of the question asked. 🚮
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Complete thread:

Internal Standard Variation Dr. Harish L. Rao 2007-09-08 14:16 [Bioanalytics]
- Internal Standard Variation Helmut 2007-09-08 16:43
  - Internal Standard Variation Ohlbe 2007-09-08 22:32
    - Internal Standard VariationHelmut 2007-09-09 00:37
      - Internal Standard Variation Charl 2007-09-09 12:25
        
        Internal Standard Variation Jaime_R 2007-09-09 13:13
        
        IS vs ES methods Helmut 2007-09-09 15:05
        Internal Standard Variation Charl 2007-09-10 10:10
        
        Internal Standard Variation Jaime_R 2007-09-10 18:58
      - Internal Standard Variation Ohlbe 2007-09-09 23:20
        
        Internal Standard Variation Helmut 2007-09-10 20:33
- Internal Standard Variation vamshi 2008-08-14 12:33
  - Internal Standard Variation ElMaestro 2008-08-14 14:09
    - Internal Standard Variation Helmut 2008-08-14 20:28
      - Internal Standard Variation ElMaestro 2008-08-15 16:51
        
        Internal Standard Variation Helmut 2008-08-15 17:29