Bioequivalence and Bioavailability Forum

Dear all,

I would be very grateful if anyone can help me in answering my questions:

1- in ANOVA section in winnonlin 2.1 there are three sub-sections "Contrast", "Tests" and "Least Squares Means" that I don't know what I have to put there. The Bioequivalence test runs without putting anything in there.can anybody tell me about the utility of these subsections?

2- when I run the bioequivalence test in 3x3x3 study with carryover effect
Seq + Subject(Seq) + Period + Trt  + Carryovr

the program gives me the following error:

     Bioavailability Statistics

     Dependent variable: Cmax

     Alpha=0.0500  Bioequivalence Limits: (A.H.)Lower= 0.800 (A.H.)Upper= 1.200
     Transformation = NONE  Percent of Reference to Detect = 0.20
     Reference least squares mean not estimable

     Test : B             Least squares mean not estimable

     Test : C             Least squares mean not estimable

and when I eliminate the Carryover effect form the model it goes well.

Can any body help me in soving this problem?

Thank you very much in advance,