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RegisterA manifold of potential causes & a story [Bioanalytics]
Hello HS
Really strange and interesting story.
Details! Why not!
I'm trying to extract Amlodipine from plasma with following scheme.
1) 0.4ml plasma+ 50 ul IS+ 40ul 1N NaOH+ 1ml Tertiary Butyl Methyl Ether.
2) Vortex for 1min.
3) Centrifuge at 9000g for 10min.
4) Take 850ul supernatant. Dry under Nitrogen stream and reconstitute in 80ul mobile phase.
The analytical range is 0.5-12ng/ml on Fluorescence.
And thanks for the book reference.
Regards
Really strange and interesting story.
❝ Without knowing details of the method we have no clue.
Details! Why not!
I'm trying to extract Amlodipine from plasma with following scheme.
1) 0.4ml plasma+ 50 ul IS+ 40ul 1N NaOH+ 1ml Tertiary Butyl Methyl Ether.
2) Vortex for 1min.
3) Centrifuge at 9000g for 10min.
4) Take 850ul supernatant. Dry under Nitrogen stream and reconstitute in 80ul mobile phase.
The analytical range is 0.5-12ng/ml on Fluorescence.
And thanks for the book reference.
Regards
Complete thread:
- Causes of inconsistent recovery Ionsource 2012-10-08 16:53
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- A manifold of potential causes & a story Helmut 2012-10-08 19:04
- A manifold of potential causes & a storyIonsource 2012-10-09 08:50
- A manifold of potential causes & a story Helmut 2012-10-08 19:04
Ing. Helmut Schütz