Bioequivalence and Bioavailability Forum • Abnormal calibration curve

Abnormal calibration curve [Bioanalytics]

posted by ElMaestro – Denmark, 2012-09-12 12:47 (4643 d 22:02 ago) – Posting: # 9182
Views: 5,269

Hi Stdev,

❝ I have an 8-point calibration curve and have established my LLOQ after multiple successful runs. However, I am now noticing the following symptoms in my MS system: The 2-3 lowest calibrators exhibit approximately the same, near zero or zero signal, then all the others appear to be normal, for standards in mobile phase and matrix. Any ideas about what could be causing the problem?

An old column? S:N too high for the peak algorithm to quantify properly?

—
Pass or fail!
ElMaestro

Complete thread:

Abnormal calibration curve stdev 2012-09-11 22:00
- Abnormal calibration curveElMaestro 2012-09-12 10:47
- Abnormal calibration curve Helmut 2012-09-12 14:40
  - Abnormal calibration curve stdev 2012-09-12 19:10
    - Abnormal calibration curve Ohlbe 2012-09-12 19:21
      - Abnormal calibration curve stdev 2012-09-12 21:58
        
        Abnormal calibration curve Ohlbe 2012-09-12 22:32
    - Abnormal calibration curve Helmut 2012-09-12 19:26
      - Abnormal calibration curve stdev 2012-09-13 16:45