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RegisterAnticoagulant: avoid CPD [Bioanalytics]
Dear Constantinos,
I fully agree with Helmut, and particularly in the case of CPD. The problem with CPD plasma is the large volume of buffer added to blood. It basically results in a 30 % dilution of plasma compared to EDTA-coated vacutainers. This can create differences in matrix effects and in recovery, depending on your sample preparation method.
I've seen a presentation by a French inspector at the EBF meeting in Barcelona in 2009. He reported two-fold differences in internal standard response between CPD and EDTA plasma, with also differences in retention times and peak width. You can expect this issue to be raised in case of an inspection.
Regards
Ohlbe
I fully agree with Helmut, and particularly in the case of CPD. The problem with CPD plasma is the large volume of buffer added to blood. It basically results in a 30 % dilution of plasma compared to EDTA-coated vacutainers. This can create differences in matrix effects and in recovery, depending on your sample preparation method.
I've seen a presentation by a French inspector at the EBF meeting in Barcelona in 2009. He reported two-fold differences in internal standard response between CPD and EDTA plasma, with also differences in retention times and peak width. You can expect this issue to be raised in case of an inspection.
Regards
Ohlbe
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Regards
Ohlbe
Regards
Ohlbe
Complete thread:
- Anticoagulant konkous 2012-06-19 14:59
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- Anticoagulant Dr_Dan 2012-06-19 15:36
- Anticoagulant Helmut 2012-06-19 15:41
- Anticoagulant konkous 2012-06-19 16:19
- Anticoagulant: avoid CPDOhlbe 2012-06-19 17:44
- Anticoagulant: CC=QC=unknowns Helmut 2012-06-19 19:48
- Anticoagulant: CC=QC=unknowns Ohlbe 2012-06-19 21:45
- Anticoagulant: CC=QC=unknowns Helmut 2012-06-19 19:48
- Anticoagulant konkous 2012-06-22 17:10
- Supplier of bulk EDTA plasma Helmut 2012-06-23 13:00
Ing. Helmut Schütz